SUMMARY:
CMT2-related TFG mutation induces TFG haploinsufficiency within cells and drives disease by causing progressive neurite degeneration.
TITLE:
TFG mutation induces haploinsufficiency and drives axonal Charcot-Marie-Tooth disease by causing neurite degeneration
DESCRIPTION:
CONCLUSION: CMT2-related TFG mutation induces TFG haploinsufficiency within cells and drives disease by causing progressive neurite degeneration.
CONTENT:
CNS Neurosci Ther. 2022 Aug 19. doi: 10.1111/cns.13943. Online ahead of print.
ABSTRACT
AIMS: TFG-related axonal Charcot-Marie-Tooth (CMT) disease is a late-onset, autosomal dominant, hereditary motor, and sensory neuropathy characterized by slowly progressive weakness and atrophy of the distal muscles. The objective of this study was to determine the common pathogenic mechanism of TFG-related CMT type 2 (CMT2) caused by different mutations and establish a direct association between TFG haploinsufficiency and neurodegeneration.
METHODS: Three individuals carrying the TFG p.G269V mutation but with varying disease durations were studied. The effect of the p.G269V mutation was confirmed by analyzing protein samples extracted from the blood of two individuals. The functional consequences of both CMT2 mutant gene products were evaluated in vitro. The effect of TFG deficiency in the nervous system was examined using zebrafish models and cultured mouse neurons.
RESULTS: Overexpression of p.G269V TFG failed to enhance soluble TFG levels by generating insoluble TFG aggregates. TFG deficiency disrupted neurite outgrowth and induced neuronal apoptosis both in vivo and in vitro and further impaired locomotor capacity in zebrafish, which was consistent with the phenotype in patients. Wnt signaling was activated as a protective factor in response to TFG deficiency.
CONCLUSION: CMT2-related TFG mutation induces TFG haploinsufficiency within cells and drives disease by causing progressive neurite degeneration.
PMID:35986567 | DOI:10.1111/cns.13943
SOURCE:
CNS neuroscience & therapeutics
TAGS:
TFG
CATEGORY:
Research
SUBCATEGORY:
n/a
DATE – PUBLISHED:
2022-08-20T06:39:39Z
DATE – DOI: 2022-08-20T06:39:39Z
DATE – PUBMED: 2022 Aug 19
DATE OUTPUT MATCHED: True
DATE – ADDED:
Sat, 20 Aug 2022 06:00:00 -0400
DATE – RETRIEVED:
08/20/22 08:59AM
2022-08-20T08:59:04-04:00
FEATURED IMAGE:
Media Uploaded (image/png)
IDENTIFIER:
pmid:35986567,doi:10.1111/cns.13943
PUBMED ID:
pubmed:35986567
DOI:
10.1111/cns.13943
LINK – PUBMED:
https://pubmed.ncbi.nlm.nih.gov/35986567/
LINK – DOI:
https://doi.org/10.1111/cns.13943
LINK – PUBLISHER:
https://onlinelibrary.wiley.com/doi/10.1111/cns.13943
REFERENCES:
CMT Treatment Report, Urgent Research, 2022-08-20T08:59:04-04:00, https://www.cmttreatmentreport.com.
