SUMMARY:
HC dysfunction may be involved in the pathogenesis of CMT1X.
TITLE:
Structures of wild-type and selected CMT1X mutant connexin 32 gap junction channels and hemichannels
DESCRIPTION:
In myelinating Schwann cells, connection between myelin layers is mediated by gap junction channels (GJCs) formed by docked connexin 32 (Cx32) hemichannels (HCs). Mutations in Cx32 cause the X-linked Charcot-Marie-Tooth disease (CMT1X), a degenerative neuropathy without a cure. A molecular link between Cx32 dysfunction and CMT1X pathogenesis is still missing. Here, we describe the high-resolution cryo-electron cryo-myography (cryo-EM) structures of the Cx32 GJC and HC, along with two…
CONTENT:
Sci Adv. 2023 Sep;9(35):eadh4890. doi: 10.1126/sciadv.adh4890. Epub 2023 Aug 30.
ABSTRACT
In myelinating Schwann cells, connection between myelin layers is mediated by gap junction channels (GJCs) formed by docked connexin 32 (Cx32) hemichannels (HCs). Mutations in Cx32 cause the X-linked Charcot-Marie-Tooth disease (CMT1X), a degenerative neuropathy without a cure. A molecular link between Cx32 dysfunction and CMT1X pathogenesis is still missing. Here, we describe the high-resolution cryo-electron cryo-myography (cryo-EM) structures of the Cx32 GJC and HC, along with two CMT1X-linked mutants, W3S and R22G. While the structures of wild-type and mutant GJCs are virtually identical, the HCs show a major difference: In the W3S and R22G mutant HCs, the amino-terminal gating helix partially occludes the pore, consistent with a diminished HC activity. Our results suggest that HC dysfunction may be involved in the pathogenesis of CMT1X.
PMID:37647412 | DOI:10.1126/sciadv.adh4890
SOURCE:
Science advances
TAGS:
HC
CATEGORY:
Research
SUBCATEGORY:
n/a
DATE – PUBLISHED:
2023-08-30T18:00:33Z
DATE – DOI: 2023-08-30T18:00:33Z
DATE – PUBMED: 2023 Aug 30
DATE OUTPUT MATCHED: True
DATE – ADDED:
Wed, 30 Aug 2023 06:00:00 -0400
DATE – RETRIEVED:
08/30/23 06:38PM
2023-08-30T18:38:11-04:00
FEATURED IMAGE:
Media Uploaded (image/png)
IDENTIFIER:
pmid:37647412,doi:10.1126/sciadv.adh4890
PUBMED ID:
pubmed:37647412
DOI:
10.1126/sciadv.adh4890
LINK – PUBMED:
https://pubmed.ncbi.nlm.nih.gov/37647412/
LINK – DOI:
https://doi.org/10.1126/sciadv.adh4890
LINK – PUBLISHER:
https://www.science.org/doi/10.1126/sciadv.adh4890
REFERENCES:
CMT Treatment Report, Urgent Research, 2023-08-30T18:38:11-04:00, https://www.cmttreatmentreport.com.
