SUMMARY:
A DRG explant culture is a scientifically, ethically, and economically more practical option than a co-culture system for studying myelin dynamics, myelin sheath formation, and demyelinating disease.
TITLE:
Advantages of Adult Mouse Dorsal Root Ganglia Explant Culture in Investigating Myelination in an Inherited Neuropathic Mice Model
DESCRIPTION:
A co-culture of neurons and Schwann cells has frequently been used to investigate myelin sheath formation. However, this approach is restricted to myelin-related diseases of the peripheral nervous system. This study introduces and compares an ex vivo model of adult-mouse-derived dorsal root ganglia (DRG) explant, with an in vitro co-culture of dissociated neurons from mouse embryo DRG and Schwann cells from a mouse sciatic nerve. The 2D co-culture has disadvantages of different mouse isolation…
CONTENT:
Methods Protoc. 2022 Jul 22;5(4):66. doi: 10.3390/mps5040066.
ABSTRACT
A co-culture of neurons and Schwann cells has frequently been used to investigate myelin sheath formation. However, this approach is restricted to myelin-related diseases of the peripheral nervous system. This study introduces and compares an ex vivo model of adult-mouse-derived dorsal root ganglia (DRG) explant, with an in vitro co-culture of dissociated neurons from mouse embryo DRG and Schwann cells from a mouse sciatic nerve. The 2D co-culture has disadvantages of different mouse isolation for neurons and Schwann cells, animal number, culture duration, and the identification of disease model. However, 3D DRG explant neurons and myelination cells in Matrigel-coated culture are obtained from the same mouse, the culture period is shorter than that of 2D co-culture, and fewer animals are needed. In addition, it has simpler and shorter experimental steps than 2D co-culture. This culture system may prove advantageous in studies of biological functions and pathophysiological mechanisms of disease models, since it can reflect disease characteristics as traditional co-culture does. Therefore, it is suggested that a DRG explant culture is a scientifically, ethically, and economically more practical option than a co-culture system for studying myelin dynamics, myelin sheath formation, and demyelinating disease.
PMID:35893592 | DOI:10.3390/mps5040066
SOURCE:
Methods and protocols
TAGS:
DRG
CATEGORY:
Research
SUBCATEGORY:
n/a
DATE – PUBLISHED:
2022-07-25T06:16:00Z
DATE – DOI: 2022-07-25T06:16:00Z
DATE – PUBMED: 2022 Jul 22
DATE OUTPUT MATCHED: True
DATE – ADDED:
Wed, 27 Jul 2022 06:00:00 -0400
DATE – RETRIEVED:
07/27/22 07:56AM
2022-07-27T07:56:25-04:00
FEATURED IMAGE:
Media Uploaded (image/png)
IDENTIFIER:
pmid:35893592,doi:10.3390/mps5040066
PUBMED ID:
pubmed:35893592
DOI:
10.3390/mps5040066
LINK – PUBMED:
https://pubmed.ncbi.nlm.nih.gov/35893592/
LINK – DOI:
https://doi.org/10.3390/mps5040066
LINK – PUBLISHER:
https://www.mdpi.com/2409-9279/5/4/66
REFERENCES:
CMT Treatment Report, Urgent Research, 2022-07-27T07:56:25-04:00, https://www.cmttreatmentreport.com.
